Given the neurodegenerative phenotypes we observed along with our transcriptomic data demonstrating altered NMJ dynamics with Bmal1 deletion, it would be interesting to explore functional changes in acetylcholine and AChE levels in clock-disrupted spinal cord cholinergic neurons, especially in relation to changes in the rhythmically expressed ALS-linked RBPs (i.e., Fus, Hnrnpa2b1, Atxn2, Taf15, and Ncbp1). Here, CLOCK is linked to amyotrophic lateral sclerosis.