We uncovered differentially spliced genes with multiple transcript variants in cholinergic neurons with Bmal1 loss, including ALS-associated genes Stk16 (at ZT8) and Atg10 (at ZT20), both of which underwent multiple exon skipping events (Fig 7B) (Lee et al, 2015; Zhao et al, 2017; Wang et al, 2019; García-García et al, 2021). Here, BMAL1 is linked to amyotrophic lateral sclerosis.