The platform enabled rapid, label-free mapping of metabolic activity and vascular heterogeneity, while spectral unmixing further allowed the quantification of endogenous biomarkers such as cytochrome c oxidase (CCO), hemoglobin derivatives, and lipids, revealing molecular patterns consistent with histopathological tumor grading according to the 2021 WHO classification. The gene discussed is RYR1; the disease is neoplasm.