Through the analysis of target genes and the functional enrichment of miR-20b, as well as the correlation of target genes with M1 macrophage infiltration, radiosensitivity, and HPV status, CCND1 emerged as a prominent gene, which had been identified as the key gene through which miR-20b regulates radiosensitivity in HPV+ HNSCC. This evidence concerns the gene CCND1 and head and neck squamous cell carcinoma.