Western blot analysis further revealed that, compared to PRNP silenced pancreatic cancer cells, the PRNP protein expression level in Ferrostatin-1 - treated silenced cells exhibited an upward trend, providing robust protein level evidence for the relationship between Ferrostatin-1 and PRNP gene expression (Figures 9B, C). This evidence concerns the gene PRNP and familial pancreatic carcinoma.