The TCGA database analysis of the expression of 65 cuproptosis-related genes revealed that compared with normal patients, 16 genes (NLRP3, PDHA1, CDKN2A, GCSH, LOXL2, ULK1, VEGFA, ATOX1, DNA2, NTHL1, POLE, POLD1, PPRAT, RTEL1, CDK5RAP1, HSPA1B) were upregulated in READ patients. The gene discussed is NLRP3; the disease is reading.