The diagnostic performance of these biomarkers was assessed using ROC analysis, which showed that the markers had an AUC of 0.6788, 0.9894, 0.9900, 0.9913, 0.9999, 0.9937 and 0.9929 for fetuin-A, klotho, BTP, Uromodulin, ADMA, NGAL and B2M in CKD detection, respectively, as described in Table-II. This evidence concerns the gene AHSG and chronic kidney disease.