Notably, however, the frequency of TNF-α, and IFN-γ producing T cells was increased for ABE-TILs upon co-culture with autologous tumor lines naturally expressing TIGIT or TIM3-specific ligands (i.e., CD112 and CD155 and galectin-9, respectively) (Figures 4B and S3). This evidence concerns the gene LGALS9 and neoplasm.