Compared to the control group and blank serum group, we observed a notable increase in the expression of autophagy-related proteins LC3B-II, p-UKL1, and Beclin-1 within the SGD-containing serum group (P < 0.01), while a marked reduction in Bcl-2 expression (P < 0.01). This evidence concerns the gene BECN1 and specific granule deficiency.