As expected, Ile gavage-DSS mice with IKK-16 treatment displayed rescued body weight, decreased DAI values, higher colon length, and lower colonic HAI scores compared with Ile gavage-DSS mice (Figures 6A–E), indicating that the NF-κB signaling pathway played a critical role in Ile aggravating colitis. This evidence concerns the gene NFKB1 and colitis.