The initially included variables were: gender (male or female), age, BMI, Smoke (yes or no), Drink (yes or no), Prior history of biliary disease (yes or no), DM (yes or no), Hypertension (yes or no), Underlying heart disease (yes or no), Renal insufficiency (yes or no), Fever (yes or no), WBC, HB, PLT, CRP, PCT, PT, AST, ALT, γ-GT, ALP, ALB, TBIL, Creatinine, Blood culture (yes or no), Broth culture (yes or no), Abscess location, Single abscess (yes or no), Abscess diameter, Pleural effusion (yes or no), and Ascites (yes or no). This evidence concerns the gene F2 and Ascites.