To determine the role of FABP4 in MASLD-HCC, we subjected Fabp4-KO (Fabp4–/–) male mice and their WT littermates to N-nitrosodiethylamine (DEN) treatment at 2 weeks of age and fed them a high-fat diet (HFD) at 6 weeks of age for 29 weeks to induce MASLD-HCC formation (Figure 3A). The gene discussed is FABP4; the disease is hepatocellular carcinoma.