Furthermore, we observed about a 4-fold increase in the secretory level of FABP4 (a mean of 95 ng/mL) in CAACM compared with that in ADCM based on the conditioned media collection shown in Supplemental Figure 1, B and C. To investigate the functional role of adipocyte-derived FABP4 in the regulation of liver CSC properties, we examined the CSC properties of HCC cells by administering recombinant human FABP4 protein (rhFABP4) at 20 ng/mL, 40 ng/mL, and 100 ng/mL, where these concentrations represent physiological levels from ADCM and CAACM. This evidence concerns the gene FABP4 and hepatocellular carcinoma.