To explore the effects of AML cell infiltration on hematopoiesis, we analyzed alterations in multiple hematopoietic compartments within the BM, and 2 EMH sites — the liver and spleen — using a nonirradiated AML mouse model driven by the human MLL-AF9 fusion protein (28) (Supplemental Figure 1A; supplemental material available online with this article; https://doi.org/10.1172/JCI193082DS1). Here, MLLT3 is linked to acute myeloid leukemia.