TFRC and acute myeloid leukemia: To determine whether heterogeneous erythroblast subpopulations exist in the spleens of AML mice and identify which subpopulations mainly contribute to AML progression, we performed single-cell RNA-Seq (scRNA-Seq) on CD45–CD71+TER119+CD44+ erythroblasts (total Erys) isolated from the spleens of CTRL mice and advanced-stage AML mice using the 10x Genomics platform (Figure 2A).