Subsequent limiting dilution assays with YFP+ leukemia cells from the primary transplant revealed that the LIC frequency was 1 in 11 of Asic3-null leukemia cells, which was approximately 9-fold higher than that in WT control cells (n = 1 in 93 cells) (Figure 1I and Supplemental Table 1). This evidence concerns the gene ASIC3 and leukemia.