To be consistent with the study in the murine AML model, ASIC3 was knocked down in THP-1, U937, MOLM-13, and Kasumi-1 cells by the validated shRNAs targeting ASIC3 (sh-ASIC3_1-2), which drastically enhanced their proliferation in vitro (Supplemental Figure 10, B–I). Here, ASIC3 is linked to acute myeloid leukemia.