The results demonstrated distinct expression patterns across groups: in the CF group, col1a, lcat, itgal, pecam1, eef1a1, and rabb3b exhibited significantly higher relative expression levels compared to other groups (p < 0.05), whereas pla2g12b, pfkfb4, and rptor showed significantly lower expression levels (p < 0.05). The gene discussed is PFKFB4; the disease is cystic fibrosis.