Hepatic mRNA expression of the fibrotic and/or inflammatory markers collagen type 1 alpha 1 (Col1a1) and transforming growth factor beta 1 (Tgfb1) increased similarly in Plin4+/+ and Plin4−/− mice fed WD (Fig. 4D), suggesting comparable deteriorating effects on liver health with WD in Plin4+/+ and Plin4−/− mice. This evidence concerns the gene PLIN4 and Wilson disease.