We then performed in vivo tumor formation assay by pairwise injecting SIRT3-overexpressed PANC1 cells and the vector-transfected PANC1 cells as the control (NC) to each side of the same mice, or by inoculating PANC1 cells with shRNA-mediated stable knockdown of SIRT3 in comparison with control PANC1 cells (Fig. 3F). The gene discussed is SIRT3; the disease is neoplasm.