Histological analysis of coronal sutures collected from WT and cKO mice at P1, P7, P14, and P28 revealed that Mapk14f/+ cKO mice showed no significant alleviation in premature suture fusion, whereas Mapk14f/f cKO mice maintained patent sutures (Fig. 8g), indicating that Mapk14 ablation in Prrx1+ cells effectively ameliorated craniosynostosis, with concomitant resolution of cranial deformities as confirmed by μCT scanning (Fig. 8h). Here, PRRX1 is linked to craniosynostosis.