There is a downregulation of genes coding for GLS, GOT2, and GPT2 enzymes in CKD3/4 versus control in the cortex (all P < 0.05, Supplementary Fig. 5B), and in both CKD groups in the medulla (all P < 0.01, Supplementary Fig. 5C). The gene discussed is GPT2; the disease is chronic kidney disease.