Finally, the targeting of ALKBH5 using knockout or vesicle-like nanoparticles (VNP)-encapsulated siRNA together with chemotherapy synergistically inhibits the growth of colorectal CSCs and PDOs and Alkbh5 knockout mice, suggesting that ALKBH5 is a potential therapeutic target in CRC. The gene discussed is ALKBH5; the disease is colorectal carcinoma.