BDNF and Anxiety: Pathway analysis on genes annotated to these sex-dependent regions show male-biased enrichment for pathways related to BDNF signalling (Fig. 3e), in line with male-biased expression changes related to Neuropeptide Signaling (Fig. 2d), and female-biased enrichment for pathways related to synapse regulation and, importantly, anxiety-related behaviour (Fig. 3e), consistent with the female transcriptional enrichment for Behavioral Fear Response genes (Fig. 2d) and the effects of Egr1 on anxiety-related behaviour in females (Fig. 1h).