This cytotoxic effect of eosinophils on chordoma cells could be inhibited by blocking TNF-α via neutralizing antibodies (Certolizumab pegol, MCE, HY-P9953), which resulted in a 22% decrease in apoptosis rate at 5 μg/ml and 41% decrease at 10 μg/ml (Fig. 4B). Here, TNF is linked to chordoma.