Next, we evaluated the antitumor effects of eosinophils on chordoma cells, and the results showed that eosinophils could inhibit chordoma cell proliferation by inducing apoptosis and secreting inflammatory cytokines (TNF-α, IL-2 and IFN-γ), and the apoptotic effects could be reversed by blocking TNF-α via neutralizing antibodies, suggesting that eosinophils could inhibit chordoma cell proliferation by inducing apoptosis, and TNF-α may play a role in chordoma cell death induced by eosinophils. The gene discussed is IL2; the disease is chordoma.