ACTA1 and hepatocellular carcinoma: Notably, in the current study, at a low concentration of 3.125 μg/ ml of ASmA and ATgA and a low concentration of 2.5% for T. gondii IgG-positive sera, a biphasic effect was demonstrated on HepG2 HCC cells and HT-29 CRC cells, respectively, where significant cellular proliferation was observed.