Furthermore, to explore the synergistic function of CMTM6 and WWP2 in HCC progression, we transfected a CMTM6 expression vector into WWP2-knockdown HuH-7 cells to generate a model with concurrent CMTM6 overexpression and WWP2 knockdown (Supplementary Fig. 2A). The gene discussed is WWP2; the disease is hepatocellular carcinoma.