Although previous research suggests Miro1 loss does not affect the cytosolic or mitochondria calcium concentrations in mouse embryonic fibroblasts during development, ablation of Miro1 has been found to decrease endoplasmic reticulum-mitochondrial tethering which alters calcium buffering and contributes to increasing autophagy and mitophagy leading to neuronal death in neurodegenerative diseases such as Parkinson's and Alzheimer's diseases (Berenguer-Escuder et al., 2020; Kam et al., 2020). Here, RHOT1 is linked to early-onset autosomal dominant Alzheimer disease.