CPSF6 and infection: We challenged these HT1080-CPSF6-mNGreen cells with VSV-G pseudotyped HIV-1 NL4-3 dEnv encoding either a wild-type (WT) capsid or a capsid mutant that is deficient in CPSF6 binding (N74D) at a multiplicity of infection (MOI) of 10 in technical triplicate.