By stratifying our analysis based on IHC results indicating high or low TIL infiltration, we sought to elucidate whether differences in the early explant culture values, such as percentages of CD3+ T cells, CD4+, CD8+ T cell subsets, CD8/CD4 ratio, cytokine production and TCR repertoire features, could be associated with the degree of TIL infiltration observed in the tumor tissue. The gene discussed is CD4; the disease is neoplasm.