Similarly, in high-fat diet–induced (HFD induced) pre-diabetic rats subjected to cardiac ischemia–reperfusion injury, administration of the mitochondrial fusion promoter M1 (2 mg/kg), either alone or in combination with the Drp1 inhibitor Mdivi-1 (1.2 mg/kg), significantly reduced mitochondrial dynamics imbalance, ROS generation, swelling, and membrane depolarization, which collectively decreased infarct size and arrhythmias while improving left ventricular function [177]. The gene discussed is DNM1L; the disease is myocardial ischemia.