To systematically evaluate the impact of down-regulating CaMKKβ expression on autophagy in NaB-induced colorectal cancer cells, we established six experimental groups: the negative control group for CaMKKβ (NC), the positive control group with NaB treatment (NC combined with NaB treatment), CaMKKβ siRNA-1, CaMKKβ siRNA-1 combined with NaB treatment, CaMKKβ siRNA-2, and CaMKKβ siRNA-2 combined with NaB treatment. This evidence concerns the gene CAMKK2 and colorectal cancer.