Moreover, qRT-PCR revealed a dose-dependent upregulation of p53 target genes Puma and Zmat3 and to a lesser extent Cdkn1a (p21) and Bax in all Trp53R210X/R210X T-lymphoma cell lines but not in T-lymphoma cell lines derived from Trp53R172H/R172H mice upon treatment with G418 (Fig. 7E; Supplementary Fig. S7B), confirming that full-length p53 induced by G418 is functional as transcription factor. Here, BBC3 is linked to lymphoma.