CCR2 and Alzheimer disease: To confirm that CCR2 and glycocalyx HSPGs are expressed in iBECs after 9 days in subculture, we isolated mRNA and performed qPCR in two independent differentiations of iBECs, alongside human brain microvessels (hBMVs) isolated from three different adult donors (age range 30–60) without a clinical diagnosis of dementia or brain pathology consistent with Alzheimer’s disease.