Meanwhile, diagnostic ROC curves showed that, except for LILRA5 which had moderate diagnostic efficacy, HNRNPL and AGBL3 both exhibited high diagnostic efficacy in Osteoporosis and stroke, with area under the curve (AUC) values greater than 0.85 (Fig 4F and G), More relevant indicators for diagnosing AUC, such as Sensitivity, Specificity, Youden index, Optimal threshold, and Accuracy, are shown in Table 4. This evidence concerns the gene HNRNPL and Stroke.