Previous studies have found that Nrf2 pathway can regulate ferroptosis-related proteins [23], and this study found that GSPE can inhibit ferroptosis, but whether GSPE inhibits ferroptosis by activating the Nrf2 pathway is not clear, in order to explore the role of GSPE in the renal tissues of DKDrats, this study examined the expression of the Nrf2 pathway-related proteins in the renal tissues of rats with DKD in each group. Here, NFE2L2 is linked to diabetic kidney disease.