TNF and colitis: As well, WT(PFKFB3fl/flLyz2-Cre) mice developed less severe colitis compared to WT(PFKFB3fl/fl) groups, showing reduced weight loss (Fig. 5a), lower DAI scores (Fig. 5b), longer colon length (Fig. 5c), diminished histopathology and intestinal damage (Fig. 5d and e), and lower inflammatory cytokine levels of Il1β, Il6, and Tnfα (Fig. 5f and g).