Even in neutrophil-depleted mice, PFKFB3 deficiency continued to alleviate colitis phenotype, as indicated by reduced body weight loss, longer colon length, and fewer colon-infiltrating immune cells (Fig. S5a through e), suggesting that the deletion of PFKFB3 in macrophages negatively regulates the colitis phenotypes. This evidence concerns the gene PFKFB3 and colitis.