TMEM59L_KD showed 92% reduction in mRNA expression (0.08-fold relative to Control_KD), while TMEM59L_OE demonstrated 384-fold increase in TMEM59L mRNA levels compared to Control_OE.We firstly treated two colorectal cancer cell lines HCT116 and LoVo with siRNA-TMEM59L with four different sites small interfering RNA and the siRNA interference effect was effective (Figure 2A). The gene discussed is TMEM59L; the disease is colorectal cancer.