WB and qPCR analysis revealed that Galunisertib reversed the upregulation of LC3II/I, p62, and downregulation of LAMP2 in the lung tissue of silicosis mice, indicating that the accumulation of autophagic substrates and impairment of lysosomal function in the lung tissue of silicosis mice were improved by Galunisertib intervention, which is consistent with our previous research results (Tan et al., 2023; Tan et al., 2020a). Here, SQSTM1 is linked to silicosis.