Through integrated multi-omics analysis and machine learning approaches, this study has successfully identified CXCL2 and MLF1 as novel diagnostic biomarkers for DN, laying a crucial foundation for developing innovative diagnostic and therapeutic strategies.CXCL2 (C-X-C Motif Chemokine Ligand 2) is a small protein that is secreted by cells such as active monocytes, neutrophils, and macrophages. The gene discussed is XCL2; the disease is liver dysplastic nodule.