By applying the TIMER method, we examined the association between tumor purity and the expression of MB, TP53, CENPA, BUB1B,ZWINT and MAD2L1 genes in HNSC (MB: Rho = −0.099, p = 2.82e-02; CENPA: Rho = 0.235, P = 1.38e-07; TP53: Rho = 0.089, P = 4.88e-02; BUB1B: Rho = 0.226, P = 3.87e-07; MAD2L1: Rho = 0.24; P = 7.28e-08; ZWINT: Rho = 0.219,P = 9.16e-07). The gene discussed is MAD2L1; the disease is neoplasm.