For instance, the Bu Shen Hua Zhuo Formula (BSHZF) reduced hyperandrogenism, fasting glucose, and IR in letrozole-induced PCOS rats while restoring microbial α-diversity, enriching Lactobacillus and SCFA-producing bacteria, and suppressing the TLR4/NF-κB inflammatory pathway by lowering serum LPS (21). The gene discussed is TLR4; the disease is polycystic ovary syndrome.