CNR1 and Anxiety: To further validate the critical role of eCB in the vAI-PrL and dAI-ovBNST circuits for modulating headache and comorbid anxiety, we combined systemic intraperitoneal administration of JZL184 with local microinjections of the CB1R antagonist NESS0327 into PrL and ovBNST, respectively, followed by behavioral assessment in mice (Fig. 8A to C and K to M).