PRL and Anxiety: In comparison with vehicle-injected mice, ISDN-injected mice showed a remarkable elevation of eCB release of vAI neuronal projections in PrL during the Von Frey test (Fig. 4J to L) but not in the EPM test (Fig. 4N to P), suggesting that the eCB of vAI-PrL circuit is likely involved in headache rather than anxiety.