Given that our RNAscope analysis revealed dense Cnr1 mRNA expression (encoding CB1R) in both PrL-projecting and ovBNST-projecting AI glutamatergic neurons (Fig. 4A to D), it is possible that eCB signaling within the vAI-PrL and dAI-ovBNST circuits regulates the comorbidity of headache and anxiety. This evidence concerns the gene PRL and Headache.