PRL and Anxiety: Subsequently, we used a chemogenetic strategy to examine whether a selective inhibition of vAI-PrL and dAI-ovBNST circuits blocked ISDN induction of headache and anxiety by bilaterally injecting AAV2/retro-hSyn-Cre-EGFP into PrL or ovBNST and AAV2/9-hSyn-DIO-hM4Di-mCherry (AAV2/8-hSyn-DIO-mCherry was used as the control) into AI (Fig. 3D, E, M, and N).