Notably, behavioral assays revealed that in mice with ISDN-induced chronic headache and anxiety comorbidity, systemic CNO-evoked chemogenetic inhibition of PrL-projecting vAI neurons attenuated cephalic cutaneous allodynia (Fig. 3F) without altering anxiety-like behaviors in the OFT (Fig. 3G to I) or EPM (Fig. 3J to L) tests. Here, PRL is linked to headache disorder.