Surprisingly, behavioral testing revealed that knockdown of DAGLα in either the vAI-PrL or dAI-ovBNST circuit ‌failed to block cephalic cutaneous allodynia or anxiety-like behavior in ISDN-injected mice (Fig. 5G to M and T to Z), suggesting that ‌reducing eCB levels‌ within vAI-PrL and dAI-ovBNST synapses ‌does not affect‌ headache or comorbid anxiety. The gene discussed is DAGLA; the disease is Anxiety.