To further characterize the functional properties of TCR-engineered T cells and to elucidate their effector molecule secretory profile, a critical indicator of their antigen-specific activation and antitumor potential, we analyzed their secretome compared to non-transduced T cells (LV-neg) following 16–18 hours of co-culture with HER2/neu-expressing SK-Mel-37 tumor cells, using the LEGENDplex multiplex cytokine profiling assay. This evidence concerns the gene ERBB2 and neoplasm.