RB1 and cancer: T24 is aligned with the FGFR3/CCND1 molecular subtype of muscle-invasive bladder carcinoma (HRAS-driven), Hedgehog-dependent (relies on the presence and activity of Hedgehog ligands to promote cancer growth, stemness, and drug resistance), glycolytic but metabolically less extreme than HT1376 cell line, which harbors genetic alterations that suggest a more invasive and metastatic phenotype (TP53/RB1/PTEN loss), is highly glycolytic and Hedgehog-independent [52,53,54].