While the unmodified mRNA OVA vaccine elicited antitumor effects characterized by robust infiltration of CD40+ DCs and OVA-specific IFN-γ secreting T cells, the vaccination with pseudo-uridine modified mRNA greatly decreased immunogenicity (decreased IFN-γ production and TNFα-producing CD8+ T cells) in spite of the highest translation efficiency, resulting in increased tumor growth and number of lung metastases [195]. The gene discussed is CD8A; the disease is neoplasm.