For spatially resolved single-cell RT-qPCR analysis, we curated a panel of 48 genes, partially derived from a whole-transcriptomic screen of over 1000 highly expressed GSC-specific genes (Figure 4A and Supplementary Table S1), ensuring relevance to glioma invasion (VIM, ZEB1/2, FSCN1, CDH2, SPARC, FN1, LOX), stemness (SOX2, NES, GFAP, PROM1, FUS, YAP1, NOTCH1), and cell cycle regulation (CDKN2A, CCND1, E2F1, MCM2, TOP2A, KIF2C, TP53). The gene discussed is E2F1; the disease is glioma.