Studies from Talhouk’s laboratory compared 3D cultures in Matrigel® and polyHEMA using the HMT-3522 S1 cell tumor transformation model, revealing that reduced expression of Connexin 43 (Cx43) induced delocalization of β-catenin, resulting in the loss of apical polarity exclusively in polyHEMA spheroids compared with Matrigel® cultures [66,131]. The gene discussed is GJA1; the disease is neoplasm.