Similarly, in spheroids derived from primary breast tumor tissues, no significant methylation differences were observed for SBDS ribosome maturation factor (SBDS) and transmembrane serine protease 2 (TMPRSS2) genes between 3D and 2D cultures, while the paired box 5 (PAX5) gene showed differential methylation without an assessed biological effect [5]. This evidence concerns the gene SBDS and breast neoplasm.