To determine whether the PCa cell line C4-2 would evolve similar insensitivity to IL-1 post chronic exposure, two sets of C4-2 chronic IL-1 sublines were generated by culturing cells in 0.5 ng/mL of IL-1α, IL-1β, or vehicle control for 6 months to generate, respectively, two C4-2 IL-1α sublines (C42as1 and C42as2), two C4-2 IL-1β sublines (C42bs1 and C42bs2), and two C4-2 parental control cell lines (C4-2 Par1 and C4-2 Par2). Here, IL1B is linked to posterior cortical atrophy.