After 24 h of incubation, unstimulated PBLs from AD patients released significantly lower levels of TNF-α (p(BH) = 0.026), IL-1β (p(BH) = 0.012), and IL-6 (p(BH)= 0.012) compared to PBLs from healthy controls (Table 4). This evidence concerns the gene TNF and Alzheimer disease.