Functional annotation of differentially methylated genes further revealed that hypermethylated genes were enriched in pathways including axon guidance, chemokine signaling, pluripotency stem cell signaling, long-term depression, circadian rhythm, FcγR-mediated phagocytosis, Fc epsilon RI signaling, PI3K-Akt signaling, Cushing’s syndrome, and HIF-1 signaling. The gene discussed is FCGR2A; the disease is Cushing syndrome due to macronodular adrenal hyperplasia.