Expression of six genes (ENO1, GLRX2, PLOD1, PRDX4, TAGLN2, and TMED9) was identified to be significantly upregulated in tumor tissues, exhibiting an increase in HR on breast cancer patients analyzed in the TCGA dataset using the multivariate Cox proportional hazards model and validated in an independent KMplotter dataset. The gene discussed is PLOD1; the disease is breast cancer.