A multivariate analysis revealed that when controlling TGFB2 expression, we identified six genes (ENO1, GLRX2, PLOD1, PRDX4, TAGLN2, and TMED9) that exhibited a negative correlation between mRNA expression and OS across all expression levels in the breast cancer patient, validated with an independent dataset from the KMplotter database. The gene discussed is TMED9; the disease is breast carcinoma.