According to the power analysis (two independent study groups, continuous, effect size mean, enrollment ratio = 1, type I/II-error rate, Alpha = 0.05, and Power = 80%), sample size n = 7 is sufficient for parameters such as tumor node number and total area of tumor nodes on macrophotos, all pathological changes prevalent in histological sections, lamina propria cell number in tumor and non-tumor tissue, and goblet cell highly sulfated mucin content in non-tumor tissue. The gene discussed is MUC5AC; the disease is neoplasm.