INS and gestational diabetes: Analysis of prostacyclin pathway genes prostaglandin-endoperoxide synthase 2 (PTGS2) (Figure 6C) and prostaglandin I2 synthase (PTGIS) (Figure 6D), revealed no significant differences between NG and GDM HUVECs, nor any effect of insulin stimulation, suggesting that this pathway may not be differentially regulated in GDM.